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Synthego Inc web based tool
Web Based Tool, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/web-based+tool/analysis+crispr+ice+tool/pm41945753-85-9-11
Average 86 stars, based on 1 article reviews
web based tool - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

other:

Article Title: Controlling genetic heterogeneity in gene-edited hematopoietic stem cells by single-cell expansion
Article Snippet: Fourty nanograms of purified fragment was subjected to Sanger sequencing (FASMAC, Japan) using sequencing primers (Prkdc_inner_F, huHBB_seqprimer).

Article Title: Loss of phospholipase PLAAT3 causes a mixed lipodystrophic and neurological syndrome due to impaired PPARγ signaling.
Article Snippet: The percentage of on-target recombination including insertions and deletions (indels) in the genomic DNA from the KO cells was evaluated by Sanger sequencing followed by analysis using the Synthego web-based tool (https://ice.synthego.com).

Article Title: An SPNS1-dependent lysosomal lipid transport pathway that enables cell survival under choline limitation
Article Snippet: Indels generated at the sgRNA target site were determined using Synthego ICE indel deconvolution web-based tool ( https://synthego.com ).

Article Title: Protocol for CRISPR-Cas9 genome editing of a swine cell line via electroporation
Article Snippet: We have used the web-based Inference of CRISPR Edits (ICE) tool from Synthego ( ).

CRISPR:

Article Title: The circRNA cEMSY Induces Immunogenic Cell Death and Boosts Immunotherapy Efficacy in Lung Adenocarcinoma
Article Snippet: .. 271 272 Single guide RNAs (sgRNAs) were created for CRISPR/Cas9-mediated gene 273 knockdown using the Web-based CRISPR design tool (https://design.synthego.com/) 274 and transformed into a vector lenti-CRISPR Version 2 (Plasmid 49535, Addgene).The 275 Supplemental table 4 contains a list of the sgRNA targeting sequences. ..

Knockdown:

Article Title: The circRNA cEMSY Induces Immunogenic Cell Death and Boosts Immunotherapy Efficacy in Lung Adenocarcinoma
Article Snippet: .. 271 272 Single guide RNAs (sgRNAs) were created for CRISPR/Cas9-mediated gene 273 knockdown using the Web-based CRISPR design tool (https://design.synthego.com/) 274 and transformed into a vector lenti-CRISPR Version 2 (Plasmid 49535, Addgene).The 275 Supplemental table 4 contains a list of the sgRNA targeting sequences. ..

Transformation Assay:

Article Title: The circRNA cEMSY Induces Immunogenic Cell Death and Boosts Immunotherapy Efficacy in Lung Adenocarcinoma
Article Snippet: .. 271 272 Single guide RNAs (sgRNAs) were created for CRISPR/Cas9-mediated gene 273 knockdown using the Web-based CRISPR design tool (https://design.synthego.com/) 274 and transformed into a vector lenti-CRISPR Version 2 (Plasmid 49535, Addgene).The 275 Supplemental table 4 contains a list of the sgRNA targeting sequences. ..

Plasmid Preparation:

Article Title: The circRNA cEMSY Induces Immunogenic Cell Death and Boosts Immunotherapy Efficacy in Lung Adenocarcinoma
Article Snippet: .. 271 272 Single guide RNAs (sgRNAs) were created for CRISPR/Cas9-mediated gene 273 knockdown using the Web-based CRISPR design tool (https://design.synthego.com/) 274 and transformed into a vector lenti-CRISPR Version 2 (Plasmid 49535, Addgene).The 275 Supplemental table 4 contains a list of the sgRNA targeting sequences. ..

Produced:

Article Title: Glycerophosphodiesters inhibit lysosomal phospholipid catabolism in Batten disease
Article Snippet: .. Synthego ICE indel deconvolution web-based tool ( https://synthego.com ) was then used to determine the indels that were produced at the sgRNA target site. ..

Polymerase Chain Reaction:

Article Title: DLK1 is a GATA1s-Driven Dependency and Therapeutic Target in Down Syndrome-Associated Myeloid Leukemia.
Article Snippet: The target locus was PCR-amplified with Q5® High-Fidelity DNA Polymerase (New England Biolabs, Frankfurt am Main, Germany) using primers flanking the expected Cas9 cut site region surrounding the CRISPR cut site in the genomic DNA. .. PCR products were Sanger sequenced and analyzed using a web-based tool https://ice.synthego.com/ (https://ice.synthego.com or https://apps.datacurators.nl/tide/). ..



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